Reacts against lamin a exclusively the antibody was raised against the carboxy terminus of 98 amino acids present in lamin a and absent from lamin c.
Anti lamin a c antibody.
The expected protein mass is 74 1 kda but there are 6 reported isoforms.
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.
Application anti lamin a c antibody clone 14 detects level of lamin a c has been published validated for use in ic wb.
Specific for an epitope mapping between amino acids 2 29 at the n terminus of lamin a c of human origin.
In humans this protein is encoded by the gene lmna.
Lamin a c antibodies.
Species predicted to react based on 100 sequence homology.
Lamin a c antibody 636 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml.
Since both dna blue and lamin a c red are associated with the nuclear compartment this region appears crimson in this image.
As a result this antibody recognizes lamin a but not lamin c.
The anti lamin a c antibody reveals strong nuclear lamina staining while anti lamp1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes.
Performed under reducing conditions.
The protein may also be known as lamin c dhe lamin a cdcd1 cddc and cmd1a.
Anti lamin a c antibody 636 is recommended for detection of lamin a and lamin c of mouse rat and human origin by wb ip if ihc p and fcm.
Goat anti rabbit igg h l hrp as014 at 1 10 000 dilution.
Anti lamin a c antibodies are available from several suppliers.
Western blot analysis of extracts of nci h293 cells using anti lamin a c antibody a0464.
70 kda ab8984 was used at a 1 100 dilution against human fibroblast lysate.
Raised against porcine lamin preparation.
Peptide from human lamin a c corresponding to amino acids 398 to 490.
Lamins a and c are alternatively spliced versions of the lmna gene.
These proteins are found in many different cell types in three different forms a b and c.
See machiels et al 1997.
Lamin a c antibody detects endogenous levels of total full length lamin a and lamin c 70 kda as well as the small fragment of lamin a and lamin c resulting from cleavage at aspartic acid 230 28 kda.
This antibody has been reported by an independent laboratory to detect lamin a c in human endothelial cells.
